3D Bioprinting Platform to Engineer Organoid-Based Liver Constructs
A 3D bioprinting platform was developed in the ORGANTRANS project for the controlled assembly of live spheroids containing liver organoids into a bioconstruct. This instrument is one of the ORGANTRANS technology building blocks and it enables the mixing of hydrogel bioink precursors with live spheroids. The combination of biodegradable bioinks with a sacrificial biocompatible scaffold will promote medium perfusion and vascularization through the printed bioconstruct. To maintain the bioinks in a functional state the bioprinter comprises complex temperature controls together with integrated stirring that allows keeping live spheroids in suspension during printing. Functional validation of the technology with living spheroids is conducted in a cell biology laboratory to verify the viability of printed spheroids in the bioconstruct. Thanks to 3D bioprinting platform, the ORGANTRANS project aims at the creation of functional 3D liver tissues for the replacement of liver transplantation for end-stage liver failure patients.